Instrument details

The current instrument at CAMS is: Agilent Technologies Pvt. Ltd. 6540 Ultra High Definition Accurate Mass Q TOF

The instrument specifications are as follows: (Download version)
Sub-systemDetailed specifications
MS system 6540 UHD Accurate Mass QTOF
Mass Analyzer: Quadrupole Time Of Flight (QTOF)
Ionisation Sources: ESI, APCI, APPI , ASAP
Detector: Electron multiplier
Mass accuracy within 1 ppm; m/z (z=1) less than 500
MS/MS accuracy within 2 ppm
S/N ratio of 450:1
Resolution of 42,000 at m/z 2722
High Femtogram level sensitivity, I pg at m/z 609
Dynamic range of up to five orders
Ability to scan multiple precursor ions ~50
Rapid polarity switching of 1.5 sec
Scan rates of 50 Hz
Positive and negative ion modes
Ultra high performance LC system Agilent 1260 Infinity Binary LC system
Binary pump with on-line vacuum degasser; high pressure binary mixing
Pressure operating range: 600 bar; flow rate range 0.001 to 5.0 ml/min; in 0.001 ml/min increments; flow precision< 0.07% RSD ; flow rate accuracy: 1 %
Autosampler capacity of 100 vials
Injection volume of 0.1-100 l in 0.1l increments; ability to inject up to 1800 l with multiple draw kit.
pH range 1.0-12.5
Peltier column cooling range: 10 deg C below ambient to 80 deg C; temp stability of 0.15 deg C
Nano-LC system Agilent 1260 Infinity Nanoflow LC
Flow rate between 0.01 to 4.0 l per min; Flow Precision :< 0.2 % RSD.
Autosampler has sample capacity of 2 x well plates (MTP); 100 x 2ml vial plates; 30 x 6-mL vials; 54 Eppendorf tubes (0.5/1.5/2.0 mL); Injection volume of 0.01 8 l in 0.01 l increments, up to 40 l.
Inbuilt temperature-controlled autosampler device with a range of 4-40 °C
Columns Eclipse XDB-C18 Solv Save 3.5u, 3.0x150mm: Particle size: 3.5 m, inner diameter: 3 mm, pore size: 80 Angstrom units, packing material: C18, RP18, ODS, Octadecyl, length: 150 mm
SB-Aq, 3.0x100mm, 1.8m: Particle size: 1.8 m, inner diameter: 3 mm, pore size: 80 Angstrom units, packing material: AQ, length: 100 mm
SB-C18, 2.1x100mm, 1.8m: Particle size: 1.8 m, inner diameter: 2.1 mm, pore size: 80 Angstrom units, packing material: C18, RP18, ODS, Octadecyl, length: 100 mm
HPLC-CHIP /MS interface Agilent CHIP cube interface
Microfluidic chip-based technology specifically designed for nanospray LC/MS.
Ambient operating temperature 5 40 °C
Fully integrated enclosed spray chamber and orthogonal dual electrode nanospray ion source.
Integrated camera and light source directly in spray chamber. Video output to external monochrome monitor for spray visualization
CHIPS for use with CHIP-MS
mAB Glyco CHIP :The Chip is designed for on-chip deglycosylation of monoclonal antibodies (mAbs) as well as subsequent on-chip enrichment, separation and MS based detection of cleaved N-glycans with an Agilent HPLC-Chip/MS system. Deglycosylation is based on an integrated immobilized PNGase F enzyme reactor column.
Phosphochip: Designed for phosphopeptide analysis;
Calib CHIP II: Used for MS calibration and Diagnostic CHIP
ProtID CHIP 43 II: Used for rapid protein identification
SmMol CHIP 43 II: Used for rapid analysis
FIA CHIP II: Dedicated infusion and flow injection CHIP
ProtID CHIP 150 II: Used for better separation of proteins
GC system Agilent High Resolution 7890 GC Split / Splitless capillary inlet: Split ratio of upto 7,500:1 to avoid column overload.
Column oven operating temperature range + 4 to 450°C
Temperature set point resolution 0.1°C
Supports 20 oven ramps
Maximum achievable temperature ramp of 120°C/min
Flame Ionisation Detector (FID) ; minimum detectable level <1.5 pg C/s
Linear dynamic range >107
Data rates upto 500 Hz, accommodate peaks as narrow as 10msec at half height